Nature biotechnology & Cells alive!

Nature reacts not only to physical disease, but also to moral weakness; when the danger increases, she gives us greater courage...
(by Johann Wolfgang von Goethe)

Clusters of the stem cells of pluripotent class in blood.

The experimental studies of generated progenitor cells culture were tested in eugamic mouse males (a line of BALB/c) with weight of 18-21 gram, being fed with cubed

feed in standard vivarium conditions. The experimental animals were injected intraperitoneally by 0.18-0.22 ml of the cell suspension in aseptic conditions.

1The microscopy of blood smears, performed in 48 hours after the beginning of the experiment, demonstrated the cluster generation likely from young forms of stem cells surrounded by the formed elements.

2Our hypothesis implies that by endogenous microcarriers in this case can be various organic aggregates of a blood of a spherical form, having adhesive superficial charge.

3The results obtained from blood smear microscopy enable assuming the optimum size of endogenic microcarriers – from 30 up to 70 μm.

4Considering the cytometric sizes of progenitor cells [10-12 μm] (Van Bekkum D.W. и соавторы, 1971; Van Bekkum D.W., 1990), it can be suggested that...

5...the part of cells settles down on the endogenic microcarriers during their migration through the rich vascularised peritoneum, and forms the active growth colonies of adherent monolayer.

6The presence of optimum conditions for cell growth and differentiation in blood supposes that the processes of active cell transformation take place directly in animal blood.

7The studying of this phenomenon is still going on.

8Clusters of the stem cells of pluripotent class in blood, magn. x400, Romanovsky's stain.

ULTRASTRUCTURAL MORPHOLOGY OF A BIOOBJECTS AFTER TERAHERTZ ILLUMINATION.

The presented morphometric data of the ultrastructural research testifies to the fact that mitochondria diameter by radiation by submillimeter waves with a modulation of 5,0 Hz reliably increases. At the same time, a size of mitochondria doesn't differ from the control by radiation of 5 Hz. Thus, a state of spermatozoa mitochondria by radiation by submillimeter waves with a modulation of 5,0 Hz can be explained by both an insignificant swelling of organelles and an increase of their functional load. A formation of specific microscopic outgrowths or microvilli formed by cytolemma in the middle part of spermatozoon tail is observed by radiation by submillimeter waves with a modulation of 5,0 Hz. It points at the tendency to increase of its active absorptive surface.

The influence on biological systems by electromagnetic radiation in millimeter and submillimeter range leads to a selective influence of terahertz radiation on the state of biological objects ultrastructure. A conjugacy of the ultrustructure and a cytophysiological functionality is a specificity of the response of cell models to electromagnetic radiation. Biological efficiency of submillimeter and millimeter waves depends on a modulating signal frequency and an intensity of the effecting factor. The comparative analysis of ultrastructural changes of spermatozoa in response to the influence of electromagnetic radiation with different frequency characteristics permits to reveal the universality of laws, caused similar biological effects.

1The longitudinal section of the spermatozoon middle part in the control. Conventional signs: CL – cytolemma; M – mitochondria; CR – cristae; FA – filament axoneme.

2The ultrastructure of spermatozoon mitochondria in the control. Round-shaped cristae are marked by arrows.

3The fragment of longitudinal and cross sections of the spermatozoon middle part after a laser illumination in the terahertz frequency range with a modulation of 5,0 Hz. MV - microvilli; AX – axoneme. At the same time, the appearance of microvilli (arrows) or microscopic outgrowths of cytoplasm, which are formed by cytolemma in the middle part of spermatozoon tail and which are specialized areas of cytolemma, should be noted.

4The ultrastructure of the spermatozoon middle part after a laser illumination in the terahertz frequency range with a modulation of 5,0 Hz. Microvilli (MV) formed by citolemma are marked by arrows.

5Mitochondria of spermatozoon with electronic-dense matrix after a laser illumination in the terahertz (or sub-millimeter) frequency range with a modulation of 5,0 Hz. MV - microvilli.