On the modern stage of medicine development the problem of creation of effective facilities of anticarcinogenic defence is becoming more and more actual. Actuality of development of innovative methods of finding pluripotent cultures with high vital and plastic potential is conditioned by the necessity of searching the new ways of fight against a cancer.
These methods find their application not only in oncology but also at the most different pathologies of nervous system, cardiovascular system, endocrine, immune, alimentary systems, etc. Their unique potential is concentrated in high repopulation and regenerative properties of pluripotent cultures that allow in the shortest time to recover the tissue defect and to create favorable possibilities for the normal functioning of organ or system.
The offered method of cultivation creates conditions for the receipt of autogenic culture of pluripotent class with sufficient cellular mass at the homogeneity of population by phenotype that determines its efficiency in the correction of different pathosiss. For the receipt of primary autogenic material the standard method of taking puncture is executed from the superior limbus of pelvic-iliac bone of a patient under local anesthesia in the outpatient setting. Cellular aspirate is exposed to the careful microscopic examination and then it is being cultivated on the composite agar-agar substrate.
The main feature of the method (Ukrpatent for useful model № 25184) developed by us is its ability to create in vitro «test-tube immunity» conditions. For this purpose the thiazole-containing compounds are added to nutrient solution (Ukrpatent for invention № 81883), and composite substrate is saturated with polyphenols and ions of silver.
Thus, duplex defence of the grown cells is formed without a necessity their periodic subcultivation. All of it creates conditions for the long-term growth and selective utilization of mature cell types in the cultivation dynamics. As a result we get a homogeneous at the

Self-organization and active ambition for uniformity form the sole base of our universe. Microcosm is as much similar to macrocosm, as we are similar to our own reflection in the mirror. Both spatial-temporal features and biosphere structure of macrocosm year by year reveal more and more reliable parallels to microcosm, which helps the researchers from different countries to come closer to understanding of microcosm scientific problems by creating various biological models. The basic idea of a new culture technique is the creation of relative aseptic conditions for prolonged growth of cell mass. The natural conditions of cells in Mammalia circulatory system can be taken as a model, and the essence of such model is the necessity to create in vitro the own immune system able to protect cells from external negative impacts (viruses, bacteria, fungi and other microorganisms). А number of quite suitable culture media which can reproduce cells environment similar to the whole blood conditions has been created for the cell cultivation (e.g., 2MEM or RPMY-1640). Same as for living macroorganisms, the use of antibiotics while cell cultivation is extremely undesirable due to their negative effect on the immunity. In condition of keeping aseptics within the whole period of cultivation, the controlled metamorphoses of less differentiated culture cell types into more mature cell types occurs. These processes will lead to the weakening of both adherent intercellular contacts and adherent cell contacts with substrate (for

Introduction: these works describe possibility of correction of radiation-induced immunodeficiency for experimental animals on isogenic and xenogenic models with the use of the pluripotent stem cells of Mammalians bone marrow grown in vitro. A new biotechnology is simple and inexpensive, giving large cellular mass as compared to the standard methods of cultivation and providing their relative phenotypic homogeneity. The physiological action of the found culture is shown in experiments on iso- and xeno-models with intraperitoneal introduction of cellular suspension in the conditions of progressive postradiational immunodeficiency state. The further study of this phenomenon showed the assured protecting of experimental animals from the consequences of X-ray influence and development of postradiational immunodeficiency.
Subject of the Research: Possibility of pluripotent hematopoietic stem cells (HSC) using in correction of immunodeficiency caused by X-ray irradiation.
Research Objective: The experimental elaboration of HSC cultivation biotechnology and pathogenetic substantiation of availability for HSC application in correction of immunodeficiency followed by X-ray irradiation.
Research techniques: hematological (amount of leukocytes, neutrophiles, lymphocytes); microscopy (optical, electron microscopy) for cytometric and cytomorphological identification of SC; immunological for determining of CD 34, CD 90, CD 117, CD 133, Sca-1 specific for SC and CD4+ and CD8+, for T-lymphocytes; physiological for determining of behavioral reactions and general condition of experimental animals; parametric and non-parametric statistics.
Novelty: Original methodology of selective cultivation of HSC (hemopoietic stem cell) of pluripotent class is first offered, suitable for reproducing in the conditions of medical establishments, foremost, at autogenic transplantations. The biotechnology of the intensive growing of phenotypic homogeneity culture HSC of pluripotent class is grounded, that can be used for the correction of the immunodeficiency states of different genesis, for example, caused general X-ray influence. The pathogenetic ground of such possibility is given.
Application Area: biology, genetics, medicine [surgical implant; oncology (e.g., breast cancer, leukemia etc.); immunodeficiency; immunodiagnostics (use of fibroblasts); endocrinology (e.g., diabetes, adiposis etc);